Open AccessPurification and characterization of other distinct bone-inducing factors.
Author(s) -
Elizabeth A. Wang,
Vicki Rosen,
Paul Cordes,
Rodney M. Hewick,
Mary Jo Kriz,
Deborah Luxenberg,
Barbara S. Sibley,
John M. Wozney
Publication year - 1988
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.85.24.9484
Subject(s) - in vivo , chemistry , molecular mass , guanidinium chloride , cartilage , peptide sequence , biochemistry , amino acid , in vitro , microbiology and biotechnology , biology , enzyme , anatomy , gene
We purified a factor that induces bone formation greater than 300,000-fold from guanidinium chloride extracts of demineralized bone. Fifty nanograms of highly purified protein was active in an in vivo cartilage and bone-formation assay. The activity resided in a single gel band, corresponding to a molecular mass of approximately 30 kDa, which yielded proteins of 30, 18, and 16 kDa on reduction. The partial amino acid sequence obtained from these proteins confirmed our identification of specific factors that induce new bone formation in vivo.