Open AccessLoss of intercellular junctional communication correlates with metastatic potential in mammary adenocarcinoma cells.
Author(s) -
Garth L. Nicolson,
Kim M. Dulski,
James E. Trosko
Publication year - 1988
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.85.2.473
Subject(s) - lucifer yellow , intracellular , mammary tumor , biology , lung , adenocarcinoma , pathology , cell junction , cell , gap junction , microbiology and biotechnology , medicine , cancer , biochemistry , breast cancer , genetics
A series of rat 13762NF mammary adenocarcinoma cell sublines and clones of various spontaneous pulmonary metastatic potentials from the mammary fat pads of syngeneic rats were examined for their intercellular junctional communication. Using the scrape-loading dye-transfer technique to introduce Lucifer yellow (Mr 457) into cells, we measured the abilities of 13762NF cells to transfer dye to adjacent cells. There was an excellent correlation between loss of Lucifer yellow dye transfer and spontaneous metastatic potential (average total volume of lung metastases inversely correlated to % cells coupled, r = 0.93; average total number of lung metastases inversely correlated to % cells coupled, r = 0.91). The data suggest that high metastatic potentials are closely correlated with loss of intercellular junctional communication in these malignant mammary tumor cells.