Cellular localization of soybean storage protein mRNA in transformed tobacco seeds | Zendy

Susan J. Barker | Zendy; John J. Harada | Zendy; Robert B. Goldberg | Zendy

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Cellular localization of soybean storage protein mRNA in transformed tobacco seeds

Author(s) -

Susan J. Barker,

John J. Harada,

Robert B. Goldberg

Publication year - 1988

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.85.2.458

Subject(s) - endosperm , storage protein , biology , messenger rna , cotyledon , gene , nicotiana tabacum , gene expression , beta (programming language) , embryo , in situ hybridization , microbiology and biotechnology , genetics , botany , computer science , programming language

We transformed tobacco plants with a soybean β-conglycinin gene that encodes the 1.7-kilobase β-subunit mRNA. We showed that the β-conglycinin mRNA accumulates and decays during tobacco seed development and that β-conglycinin mRNA is undetectable in the tobacco leaf. We utilizedin situ hybridization to localize β-conglycinin mRNA within the tobacco seed. β-Conglycinin mRNA is not detectable within the endosperm but is localized within specific embryonic cell types. The highest concentration of β-conglycinin mRNA is found in cotyledon storage parenchyma cells. We conclude that sequences required for embryo expression, temporal control, and cell specificity are linked to the β-conglycinin gene, and that factors regulating β-conglycinin gene expression are compartmentalized within analogous soybean and tobacco seed regions.

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