Open AccessCoordinate regulation of two genes encoding gluconeogenic enzymes by the trans-dominant locus Tse-1.
Author(s) -
Janis Lem,
Alice Chin,
Mathew J. Thayer,
Robin J. Leach,
R. E. K. Fournier
Publication year - 1988
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.85.19.7302
Subject(s) - locus (genetics) , gene , tyrosine aminotransferase , biology , phosphoenolpyruvate carboxykinase , microbiology and biotechnology , genetics , gene expression , chromosome , enzyme , enzyme inducer , biochemistry
Tissue-specific extinguisher-1 (Tse-1) is a mouse genetic locus that can repress liver-specific tyrosine aminotransferase gene expression in trans. To search for other Tse-1-responsive genes, hepatoma microcell hybrids retaining mouse chromosome 11 or human chromosome 17, containing murine Tse-1 and human TSE1, respectively, were screened for expression of liver-specific mRNAs. While most liver gene activity was unaffected in such hybrids, phosphoenolpyruvate carboxykinase and tyrosine aminotransferase gene expression was coordinately repressed in these clones. Extinction of both genes was apparently mediated by a single genetic locus that resides on human chromosome 17.