Open AccesscDNA cloning and expression of murine macrophage colony-stimulating factor from L929 cells.
Author(s) -
Martha Ladner,
Gary A. Martin,
Janelle A. Noble,
Vaughan Wittman,
M K Warren,
Michael McGrogan,
E. Richard Stanley
Publication year - 1988
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.85.18.6706
Subject(s) - complementary dna , microbiology and biotechnology , biology , amino acid , macrophage colony stimulating factor , recombinant dna , peptide sequence , signal peptide , cloning (programming) , untranslated region , clone (java method) , molecular cloning , messenger rna , gene , biochemistry , macrophage , in vitro , computer science , programming language
A 4-kilobase and a 2-kilobase cDNA clone encoding a murine macrophage colony-stimulating factor have been isolated. Except for 2 amino acid residue differences, these two clones encode the same 520 amino acid residue protein, which is preceded by a 32-amino acid residue signal peptide. The two clones, whose molecular masses correspond to the two transcripts observed in murine L929 fibroblasts, contain 3' untranslated regions that are markedly different in sequence and length. Both clones can be expressed in COS cells and the recombinant protein is active in a mouse bone marrow colony assay.