Open AccessMouse lysozyme M gene: isolation, characterization, and expression studies.
Author(s) -
Michael Cross,
Inge Mangelsdorf,
Andrew Wedel,
Rainer Renkawitz
Publication year - 1988
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.85.17.6232
Subject(s) - lysozyme , microbiology and biotechnology , complementary dna , gene , biology , gene expression , nucleic acid sequence , messenger rna , genomic dna , peptide sequence , dna , biochemistry
We have isolated and characterized both cDNA and genomic DNA of the mouse lysozyme M gene. Derivation of the amino acid sequence from the nucleotide sequences revealed six positions in the carboxyl terminus that differ from partial sequences previously published. The differential detection of specific mRNAs from the closely related lysozyme M and P genes has revealed different but overlapping tissue specificities of expression. The M gene is expressed weakly in myeloblasts, moderately in immature macrophages, and strongly in both mature macrophages and macrophage-rich tissues, while high levels of P transcripts are present only in small intestine. Sites of protein accumulation, rather than gene expression, have been identified by comparative quantitation of mRNA and enzyme levels.