Open AccessIsolation of gene fusions (soi::lacZ) inducible by oxidative stress in Escherichia coli.
Author(s) -
Tokio Kogoma,
Spencer B. Farr,
Kelly M. Joyce,
Donald O. Natvig
Publication year - 1988
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.85.13.4799
Subject(s) - escherichia coli , superoxide , paraquat , lac operon , fusion gene , microbiology and biotechnology , chemistry , hydrogen peroxide , plumbagin , oxidative stress , mutant , enterobacteriaceae , fusion protein , gene , biology , biochemistry , enzyme , genetics , recombinant dna
Mu dX phage was used to isolate three gene fusions to the lacZ gene (soi::lacZ; soi for superoxide radical inducible) that were induced by treatment with superoxide radical anion generators such as paraquat and plumbagin. The induction of beta-galactosidase in these fusion strains with the superoxide radical generating agents required aerobic metabolism. Hyperoxygenation (i.e., bubbling of cultures with oxygen gas) also induced the fusions. On the other hand, hydrogen peroxide did not induce the fusions at concentrations that are known to invoke an adaptive response. Introduction of oxyR, htpR, or recA mutations did not affect the induction. Two of the fusion strains exhibited increased sensitivity to paraquat but not to hydrogen peroxide. The third fusion strain showed no increased sensitivity to either agent. All three fusions were located in the 45- to 61-min region of the Escherichia coli chromosome.