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The complete nucleotide sequences of the cDNAs encoding the heavy- and light-chain variable regions (VH and VL) of myeloma protein W3129, an anti-alpha(1----6)-dextran with a unique combining site, have been determined. The VH region is encoded by a germ-line gene highly homologous to VH441, which also appears to be used in some anti-galactans and anti-levans. W3129 VH uses a diversity region that is not found in any of the galactan- or levan-specific antibodies and whose germ-line counterpart is unknown. The W3129 light chain differs from complete light-chain sequences thus far reported but is identical in the first 23 amino acids with NZB IgA myeloma PC118.

proceedings of the national academy of sciences of the united states of america

Nucleotide sequence of the cDNAs encoding the variable region heavy and light chains of a myeloma protein specific for the terminal nonreducing end of alpha(1----6)dextran.