Open AccessThe structural characterization of endogenous factors from Vinca rosea crown gall tumors that promote cell division of tobacco cells.
Author(s) -
David G. Lynn,
Randal H. Chen,
Kenneth S. Manning,
Henry N. Wood
Publication year - 1987
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.84.3.615
Subject(s) - vinca , gall , auxin , cytokinin , cell division , endogeny , biology , cell , cell culture , biochemistry , microbiology and biotechnology , chemistry , botany , genetics , gene
The ability of two compounds, a cytokinin and an auxin, to stimulate tobacco cell growth and differentiation has been known for greater than 30 years, but the molecular mechanism of this activation is still unknown. Previous reports of factors endogenous in crown gall tumors of Vinca rosea that could replace the cytokinin requirement in tobacco cell culture has motivated an investigation of these tissues. The optimization of a reverse-phase isolation scheme has led to the purification of sufficient material to allow for the identification of six different related compounds. The structures of two of these compounds have been assigned as a set of epimeric dehydrodiconiferyl alcohol beta-D-glycosides. The structure of these compounds suggests that they would most likely be derived from the plant cell wall.