Open AccessSimian virus 40 large tumor antigen requires three core replication origin domains for DNA unwinding and replication in vitro.
Author(s) -
Frank B. Dean,
James A. Borowiec,
Yukio Ishimi,
Sumitra Deb,
Peter Tegtmeyer,
Jerard Hurwitz
Publication year - 1987
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.84.23.8267
Subject(s) - biology , dna replication , dna , replication factor c , control of chromosome duplication , origin of replication , eukaryotic dna replication , microbiology and biotechnology , seqa protein domain , origin recognition complex , virology , genetics
Simian virus 40 (SV40) large tumor antigen (T antigen) unwinds DNA containing the SV40 origin of replication. The origin requirement for unwinding can be satisfied by the 64-base-pair SV40 core origin that supports T-antigen-dependent DNA replication both in vivo and in vitro. The core origin contains three domains with specific DNA sequence features. These include an inverted repeat, a central T-antigen binding domain, and an adenine- and thymine-rich domain containing a DNA bending focus. The domain and spacer requirements of the core origin for DNA unwinding and replication in vitro are strikingly similar to the origin requirements for DNA replication in vivo. Thus, each of the three functional domains of the core origin contributes directly to the initiation of duplex DNA unwinding by T antigen.