Open AccessSelective elimination of mRNAs in vivo: complementary oligodeoxynucleotides promote RNA degradation by an RNase H-like activity.
Author(s) -
Pramod K. Dash,
Ilana Lotan,
Michael Knapp,
Eric R. Kandel,
Philip Goelet
Publication year - 1987
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.84.22.7896
Subject(s) - xenopus , rnase h , rnase p , messenger rna , in vivo , translation (biology) , oligonucleotide , rna , biology , microbiology and biotechnology , chemistry , biochemistry , genetics , gene
Oligodeoxynucleotides lead to translation arrest of complementary mRNAs in the wheat germ translation system by a degradation of the mRNA. In an attempt to develop an effective reverse genetic approach in vivo, we demonstrate that injection of short (15- to 30-nucleotide) oligonucleotides into Xenopus oocytes leads to complete degradation of both injected and endogenous mRNAs by means of an RNase H-like activity.