Selective elimination of mRNAs in vivo: complementary oligodeoxynucleotides promote RNA degradation by an RNase H-like activity. | Zendy

Philip R. Dash | Zendy; Ilana Lotan | Zendy; Michael Knapp | Zendy; Eric R. Kandel | Zendy; Philip Goelet | Zendy

Open Access

Selective elimination of mRNAs in vivo: complementary oligodeoxynucleotides promote RNA degradation by an RNase H-like activity.

Author(s) -

Philip R. Dash,

Ilana Lotan,

Michael Knapp,

Eric R. Kandel,

Philip Goelet

Publication year - 1987

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.84.22.7896

Subject(s) - xenopus , rnase h , rnase p , messenger rna , in vivo , translation (biology) , oligonucleotide , rna , biology , microbiology and biotechnology , chemistry , biochemistry , genetics , gene

Oligodeoxynucleotides lead to translation arrest of complementary mRNAs in the wheat germ translation system by a degradation of the mRNA. In an attempt to develop an effective reverse genetic approach in vivo, we demonstrate that injection of short (15- to 30-nucleotide) oligonucleotides into Xenopus oocytes leads to complete degradation of both injected and endogenous mRNAs by means of an RNase H-like activity.

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