Identification of a transposon Tn7-dependent DNA-binding activity that recognizes the ends of Tn7. | Zendy

Robert L. McKown | Zendy; C S Waddell | Zendy; Lidia K. Arciszewska | Zendy; Nancy L. Craig | Zendy

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Identification of a transposon Tn7-dependent DNA-binding activity that recognizes the ends of Tn7.

Author(s) -

Robert L. McKown,

C S Waddell,

Lidia K. Arciszewska,

Nancy L. Craig

Publication year - 1987

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.84.22.7807

Subject(s) - transposable element , biology , transposition (logic) , genetics , dna , transposase , escherichia coli , base pair , tn3 transposon , homologous chromosome , chromosome , gene , microbiology and biotechnology , mutant , linguistics , philosophy

The bacterial transposon Tn7 is distinguished by its capacity for high-frequency transposition to a specific site in the Escherichia coli chromosome. tnsB is one of the five Tn7-encoded transposition genes. We have identified in vitro a tnsB-dependent DNA binding activity that interacts specifically with cis-acting transposition sequences at the Tn7 termini. Although the left and right termini of Tn7 are structurally distinct, each end contains several copies of a closely homologous 22-base-pair sequence. We present results indicating that this 22-base-pair repeat sequence is recognized by the tnsB-dependent binding activity.

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