Open AccessExpression and secretion of type beta transforming growth factor by activated human macrophages.
Author(s) -
Richard K. Assoian,
Barbara E. Fleurdelys,
Henry C. Stevenson,
Paul J. Miller,
David K. Madtes,
Elaine W. Raines,
Russell Ross,
Michael B. Sporn
Publication year - 1987
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.84.17.6020
Subject(s) - monocyte , secretion , transforming growth factor beta , transforming growth factor , lipopolysaccharide , transforming growth factor, beta 3 , growth factor , biology , tgf alpha , beta (programming language) , macrophage , u937 cell , concanavalin a , microbiology and biotechnology , endocrinology , medicine , chemistry , cell culture , immunology , receptor , biochemistry , in vitro , genetics , computer science , programming language
Alveolar macrophages activated with concanavalin A and peripheral blood monocytes activated with lipopolysaccharide secrete type beta transforming growth factor (TGF-beta). There is minimal TGF-beta secretion in unactivated monocytes, even though TGF-beta mRNA is expressed in these cells at a level similar to that in activated, lipopolysaccharide-treated cultures. U937 lymphoma cells, which have monocytic characteristics, also express mRNA for TGF-beta. Freshly isolated monocytes, both control and lipopolysaccharide-treated, secrete an acid-labile binding protein that inhibits TGF-beta action. We conclude the following: (i) that expression of TGF-beta mRNA is unrelated to monocyte activation, (ii) that secretion of TGF-beta is induced by monocyte activation, and (iii) that cosecretion of TGF-beta and its monocyte/macrophage-derived binding protein may modulate growth factor action. In contrast, monocytic expression of other growth factor genes, such as the B chain of platelet-derived growth factor, is not constitutive and requires activation.