Open AccessCombined proton NMR imaging and spectral analysis of locust embryonic development
Author(s) -
George Gassner,
Joost A. B. Lohman
Publication year - 1987
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.84.15.5297
Subject(s) - schistocerca , locust , desert locust , spectral imaging , nuclear magnetic resonance , embryogenesis , spectroscopy , imaging spectroscopy , imaging spectrometer , spectrometer , proton nmr , high resolution , proton , embryo , chemistry , physics , biology , optics , remote sensing , ecology , geology , microbiology and biotechnology , quantum mechanics
Images and spectra of desert locust [Schistocerca gregaria (Forskal)] embryos were collectedin vivo using a 4.7-tesla proton (1 H) NMR imaging spectrometer. The 100 × 100 × 500 μm image resolution and 125-nl localized spectral volumes were obtained within minutes of each other. The dynamics of embryonic development were slow enough that the time delay between imaging and spectral measurements is negligible. Thus, image and spectral data correspond closely and approximate real-time observations of embryonic changes. The above procedure was applied every 12 hr during the entire course of development. This analytical approach demonstrates that imaging and localized spectroscopy can be used to visualize and assess changes in embryonic water and lipid content in concert with the development of a living embryo.