Open AccessHuman cDNA clones for an alpha subunit of Gi signal-transduction protein.
Author(s) -
Patricia Bray,
A. Carter,
Vicky Guo,
Carmie Puckett,
John Kamholz,
Allen M. Spiegel,
Marshall W. Nirenberg
Publication year - 1987
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.84.15.5115
Subject(s) - complementary dna , microbiology and biotechnology , biology , g alpha subunit , cdna library , interleukin 10 receptor, alpha subunit , interleukin 5 receptor alpha subunit , alpha (finance) , homology (biology) , peptide sequence , nucleic acid sequence , amino acid , protein subunit , gene , biochemistry , medicine , construct validity , nursing , patient satisfaction
Two cDNA clones were obtained from a lambda gt11 cDNA human brain library that correspond to alpha i subunits of G signal-transduction proteins (where alpha i subunits refer to the alpha subunits of G proteins that inhibit adenylate cyclase). The nucleotide sequence of human brain alpha i is highly homologous to that of bovine brain alpha i [Nukada, T., Tanabe, T., Takahashi, H., Noda, M., Haga, K., Haga, T., Ichiyama, A., Kangawa, K., Hiranaga, M., Matsuo, H. & Numa, S. (1986) FEBS Lett. 197, 305-310] and the predicted amino acid sequences are identical. However, human and bovine brain alpha i cDNAs differ significantly from alpha i cDNAs from human monocytes, rat glioma, and mouse macrophages in amino acid (88% homology) and nucleotide (71-75% homology) sequences. In addition, the nucleotide sequences of the 3' untranslated regions of human and bovine brain alpha i cDNAs differ markedly from the sequences of human monocyte, rat glioma, and mouse macrophage alpha i cDNAs. These results suggest there are at least two classes of alpha i mRNA.