Open AccessDNA segment containing C beta 1, a gene for the constant region of the beta chain of the T-cell antigen receptor, was inserted into chromosome 6 in cells from one patient with human T-cell leukemia.
Author(s) -
Teruo Ino,
Yoshikazu Kurosawa,
Michihiro C. Yoshida,
Masami Hirano
Publication year - 1987
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.84.12.4264
Subject(s) - microbiology and biotechnology , beta (programming language) , biology , chromosome , chromosome 17 (human) , gene , chromosome 21 , gene rearrangement , genetics , computer science , programming language
DNA rearrangements that occurred in the vicinity of T-cell antigen receptor beta-chain gene clusters residing on chromosome 7 were examined in human T-cell acute lymphoblastic leukemia cells. In one patient, it was observed that, for the T-cell receptor beta-chain genes, a D beta 1-J beta 2.3 (where D is diversity and J is joining) junction was found on one chromosome, while the other chromosome kept the germ-line configuration. If this D beta-J beta junction was formed by the customary deletion mechanism, the C beta 1 gene (where C is constant) located between the D beta 1 and J beta 2.3 loci should have disappeared from this chromosome. The C beta 1 gene indeed was absent from the rearranged chromosome 7, but it was found on chromosome 6 as an inserted segment. The implications of the observations are discussed.