Open AccessMultiple genes encode the human Na+,K+-ATPase catalytic subunit.
Author(s) -
Marcia M. Shull,
Jerry B. Lingrel
Publication year - 1987
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.84.12.4039
Subject(s) - scn3a , microbiology and biotechnology , gene isoform , complementary dna , g alpha subunit , biology , gene , southern blot , interleukin 5 receptor alpha subunit , alpha (finance) , homology (biology) , genomic dna , genetics , cdna library , interleukin 10 receptor, alpha subunit , genomic library , protein subunit , restriction map , nucleic acid sequence , peptide sequence , medicine , construct validity , nursing , patient satisfaction
A human genomic library was constructed and screened with hybridization probes derived from sheep and rat cDNAs encoding the alpha and alpha(+) isoforms, respectively, of the Na+,K+-ATPase catalytic subunit. Genomic sequences spanning 150 kilobases were isolated. Four genes, designated alpha A, alpha B, alpha C, and alpha D, each 20-25 kilobases in length, were identified by restriction mapping, Southern blot hybridization analysis, and limited DNA sequencing. We present evidence that two of these genes, alpha A and alpha B, encode the alpha and alpha(+) isoforms, respectively. The other genes, alpha C and alpha D, one of which is physically linked to the alpha(+) gene, exhibit nucleotide and amino acid homology to Na+,K+-ATPase catalytic subunit cDNA sequences but do not correspond to any previously identified isoforms.