Open AccessGenomic organization of the mouse T-cell receptor beta-chain gene family.
Author(s) -
Eric Lai,
Richard K. Barth,
Leroy Hood
Publication year - 1987
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.84.11.3846
Subject(s) - cosmid , gene , biology , genetics , restriction map , microbiology and biotechnology , gene family , beta (programming language) , restriction enzyme , restriction fragment , dna , genomic dna , genome , peptide sequence , computer science , programming language
We have combined three different methods, deletion mapping of T-cell lines, field-inversion gel electrophoresis, and the restriction mapping of a cosmid clone, to construct a physical map of the murine T-cell receptor beta-chain gene family. We have mapped 19 variable (V beta) gene segments and the two clusters of diversity (D beta) and joining (J beta) gene segments and constant (C beta) genes. These members of the beta-chain gene family span approximately equal to 450 kilobases of DNA, excluding one potential gap in the DNA fragment alignments.