Open AccessThe immunoglobulin heavy chain enhancer is stimulated by the adenovirus type 2 E1A products in mouse fibroblasts.
Author(s) -
Emiliana Borrelli,
René Hen,
Christine Wasylyk,
Bohdan Wasylyk,
Pierre Chambon
Publication year - 1986
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.83.9.2846
Subject(s) - enhancer , immunoglobulin heavy chain , immunoglobulin light chain , microbiology and biotechnology , biology , antibody , recombinant dna , transcription (linguistics) , enhancer rnas , repressor , cell culture , fibroblast , transfection , transcription factor , chemistry , gene , biochemistry , genetics , linguistics , philosophy
In contrast with our previous results (Hen, R., Borrelli, E. & Chambon, P. (1985) Science 230, 1391-1394), which demonstrated that the mouse immunoglobulin heavy chain transcriptional enhancer is repressed in lymphoid cells by the products of the adenovirus type 2 E1A transcription unit, we show here that these products activate the same enhancer in mouse fibroblast L cell lines that contain stably integrated copies of a recombinant in which the enhancer is inserted upstream from the chicken conalbumin promoter. In addition, competition experiments suggest that the activity of the heavy chain enhancer may be repressed by a trans-acting factor in mouse L cells. We speculate that the E1A products may prevent the action of this cellular repressor in these cells.