Open AccessComponents of microtubular structures in Saccharomyces cerevisiae.
Author(s) -
Lorraine Pillus,
Frank Solomon
Publication year - 1986
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.83.8.2468
Subject(s) - saccharomyces cerevisiae , organelle , microtubule , yeast , spindle pole body , spindle apparatus , biology , mitosis , microbiology and biotechnology , cytoskeleton , tubulin , biochemistry , genetics , cell division , cell
Most studies of cytoskeletal organelles have concentrated on molecular analyses of abundant and biochemically accessible structures. In many of the classical cases, however, the nature of the system chosen has precluded a concurrent genetic analysis. The mitotic spindle of the yeast Saccharomyces cerevisiae is one example of an organelle that can be studied by both classical and molecular genetics. We show here that this microtubule structure also can be examined biochemically. The spindle can be isolated by selective extractions of yeast cells by using adaptations of methods successfully applied to animal cells. In this way, microtubule-associated proteins of the yeast spindle are identified.