Open AccessIsolation and characterization of a mouse interleukin cDNA clone that expresses B-cell stimulatory factor 1 activities and T-cell- and mast-cell-stimulating activities.
Author(s) -
F Lee,
Takashi Yokota,
Takeshi Otsuka,
P Meyerson,
Douglas B. Villaret,
Robert L. Coffman,
Tim R. Mosmann,
Donna Rennick,
Neal W. Roehm,
Craig A. Smith
Publication year - 1986
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.83.7.2061
Subject(s) - complementary dna , microbiology and biotechnology , biology , cdna library , mast cell , transfection , clone (java method) , signal peptide , expression cloning , cell culture , peptide sequence , interleukin 4 , interleukin 9 , dna , interleukin , gene , cytokine , biochemistry , immunology , genetics
A cDNA sequence coding for a unique mouse interleukin that expresses B-cell-, T-cell, and mast-cell-stimulating activities has been isolated from a mouse helper T-cell cDNA library. The library, constructed in the pcD expression vector, was screened by transfecting COS monkey cells with DNA pools to express the products encoded by full-length cDNA inserts. By assaying the transfected cell supernatants, we identified clones encoding a factor that stimulates T-cell and mast cell lines. This factor also induces Ia expression on resting B cells and enhances IgG1 and IgE production by B cells, two properties of B-cell-stimulatory factor 1. The DNA sequence codes for a polypeptide of 140 amino acid residues including a putative signal peptide. These results demonstrate that a single cDNA clone distinct from interleukin 2 and interleukin 3 encodes a polypeptide with multiple biological activities.