Open AccessProenkephalin mRNA in rat heart.
Author(s) -
Richard Howells,
D L Kilpatrick,
Leslie Bailey,
M Noe,
Sidney Udenfriend
Publication year - 1986
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.83.6.1960
Subject(s) - proenkephalin , messenger rna , complementary dna , biology , rna , microbiology and biotechnology , prohormone , enkephalin , northern blot , translation (biology) , chemistry , biochemistry , gene , receptor , opioid , hormone
The distribution of preproenkephalin mRNA in rat tissues was investigated using a homologous cDNA probe for detection. The heart was found to contain larger amounts of the mRNA than any other tissue including brain, which heretofore had been considered the richest source. The identity of the message in heart was verified by hybridizing RNA blots with a synthetic oligodeoxynucleotide that recognizes a different region of the preproenkephalin mRNA sequence than does the cDNA probe. The preproenkephalin mRNA extracted from both heart and brain contained approximately equal to 1500 bases. Dissection of heart revealed that essentially all of the message is contained within the ventricles. In contrast to the large amounts of preproenkephalin mRNA in rat heart, the opioid peptide contents is only 3% of the amount in brain. The rat heart may be a useful model for the investigation of translation control of protein synthesis.
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