Open AccessFrequent lambda light chain gene rearrangement and expression in a Ly-1 B lymphoma with a productive kappa chain allele.
Author(s) -
Richard R. Hardy,
Jeffery L. Dangl,
Kyoko Hayakawa,
Gina C. Jager,
L A Herzenberg
Publication year - 1986
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.83.5.1438
Subject(s) - lambda , immunoglobulin light chain , kappa , microbiology and biotechnology , gene , gene rearrangement , biology , lymphoma , allele , dna , genetics , physics , antibody , immunology , philosophy , linguistics , optics
We describe here a murine Ly-1-bearing pre-B-cell tumor that, when induced for kappa light chain expression with bacterial lipopolysaccharide, also gives rise spontaneously to a few percent of cells expressing surface lambda light chains. These lambda-positive cells have undergone DNA rearrangements involving either V lambda 1 or V lambda 2 genes. Nearly all clones of lambda-bearing cells express mu and lambda on their surface (but not kappa). However, all these lambda-positive clones continue to transcribe kappa mRNA and synthesize internal kappa chains. Further, surface lambda-positive clones show JH rearrangements on one or both heavy chain chromosomes.