Open AccessCocompartmentation of proteins and K+ within the living cell.
Author(s) -
Miklós Kellermayer,
Andrea Ludány,
K Jobst,
György Szűcs,
K Trombitás,
Carlton F. Hazlewood
Publication year - 1986
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.83.4.1011
Subject(s) - cytoplasm , intracellular , triton x 100 , chemistry , membrane , monolayer , kinetics , cell , solubilization , biophysics , biochemistry , biology , pulmonary surfactant , physics , quantum mechanics
Monolayer H-50 tissue culture cells were treated with Triton X-100 and Brij 58 nonionic detergents, and their electron microscopic morphology along with the release of the intracellular proteins and K+ were studied. Although Triton X-100 was more effective, both detergents removed the lipoid membranes within 5 min. The mobilization and solubilization of the cytoplasmic and nuclear proteins occurred much faster with Triton X-100 than with Brij 58. In Triton X-100-treated cells, the loss of K+ was complete within 2 min. The loss of K+ from the Brij 58-treated cells was complete only after 10 min and the mobilization of K+ showed sigmoid-type release kinetics. These results support the view that most of K+ and "diffusible" proteins not freely dissolved in the cellular water, but they are cocompartmentalized inside the living cell.