Human autoantibodies reactive with the signal-recognition particle. | Zendy

Westley H. Reeves | Zendy; Sanjay K. Nigám | Zendy; Günter Blobel | Zendy

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Human autoantibodies reactive with the signal-recognition particle.

Author(s) -

Westley H. Reeves,

Sanjay K. Nigám,

Günter Blobel

Publication year - 1986

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.83.24.9507

Subject(s) - autoantibody , ribonucleoprotein , signal recognition particle , immunoprecipitation , immunofluorescence , biology , cytoplasm , protein subunit , microbiology and biotechnology , heterogeneous nuclear ribonucleoprotein , k562 cells , antibody , chemistry , immunology , biochemistry , cell , signal peptide , peptide sequence , rna , gene

We describe autoantibodies reactive with the signal-recognition particle (SRP) in serum of a patient with polymyositis. The serum specifically immunoprecipitated the SRP from human erythroleukemia (K562) cell extracts. Analysis of immunoblots revealed that the serum contained autoantibodies specific for the 54-kDa protein of the SRP but had little or no reactivity with its other five proteins. Indirect immunofluorescence of human laryngeal carcinoma (HEp-2) cells confirmed that the ribonucleoprotein immunoprecipitated by this serum is found mainly in the cytoplasm. This autoimmune serum may be useful for studying the function of the 54-kDa subunit of the SRP in binding to signal sequences or in interacting with other components of the translational machinery.

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