Open AccessAssociation of La and Ro antigens with intracellular structures in HEp-2 carcinoma cells.
Author(s) -
Michael Bachmann,
W.-J. Mayet,
Heinz C. Schröder,
Karin Pfeifer,
K. H. Meyer zum Büschenfelde,
Wernér E.G. Müller
Publication year - 1986
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.83.20.7770
Subject(s) - monoclonal antibody , microbiology and biotechnology , ribonucleoprotein , antigen , antibody , immunoprecipitation , immunofluorescence , biology , epitope , chemistry , virology , rna , biochemistry , immunology , gene
Monoclonal antibodies were raised against homogeneous Ro and La antigens, two proteins associated with Ro and La ribonucleoproteins (RNPs). The specificity of the monoclonal antibodies was proven by immunoblot analysis and by immunoprecipitation. The anti-Ro antibody reacted with a Mr 95,000 protein in a mouse lymphoma cell extract and with a Mr 60,000 polypeptide in extracts from human spleen. The anti-La antibody recognized a Mr 50,000 polypeptide in the mouse L5178y cell extract. The two monoclonal antibodies precipitated RNPs that contained the typical RNA species of Ro or La RNPs. The localization of Ro and La antigen was performed by direct immunofluorescence microscopy. It was found that the anti-Ro antibody reacted with a fibrous network that behaves like cytokeratin, one of the intermediate filament systems. The anti-La antibody reacted with nuclear structures that gave a speckled-type pattern.
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