Introduction of genes into preimplantation mouse embryos by use of a defective recombinant retrovirus. | Zendy

John L.R. Rubenstein | Zendy; Jean–François Nicolas | Zendy; F Jacob | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Introduction of genes into preimplantation mouse embryos by use of a defective recombinant retrovirus.

Author(s) -

John L.R. Rubenstein,

Jean–François Nicolas,

F Jacob

Publication year - 1986

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.83.2.366

Subject(s) - provirus , retrovirus , biology , embryo , virology , recombinant dna , in vitro , virus , genome , transgene , gene , cell culture , genetics , recombinant virus , microbiology and biotechnology

Two-cell and four-cell preimplantation mouse embryos were cocultured in vitro with fibroblasts producing the recombinant retrovirus M-MuLV-neo. No wildtype helper virus was detected in these cultures. Of the embryos that survived the in vitro cultivation and the reimplantation into foster mothers, 2 of 15 that were tested contained the proviral genome. The provirus integrated as a single copy at a unique site. We estimate that approximately equal to 20% of the cells in each of the two transgenic fetuses contained the provirus.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research