Open AccessA DNA binding protein specific for an origin of replication of herpes simplex virus type 1.
Author(s) -
Per Elias,
Mike O'Donnell,
Edward S. Mocarski,
I. R. Lehman
Publication year - 1986
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.83.17.6322
Subject(s) - single stranded binding protein , biology , replication protein a , dna replication , microbiology and biotechnology , dna , dna binding protein , seqa protein domain , herpes simplex virus , replication factor c , hmg box , virology , eukaryotic dna replication , virus , genetics , gene , transcription factor
We have identified a protein that binds specifically to an origin of replication (oris) of the herpes simplex virus type 1 genome. The oris binding protein, detectable only in nuclear extracts of infected cells, shows the same time course of appearance as the herpesvirus-induced DNA polymerase and the DNA binding protein ICP8. The partially purified oris binding protein generates a DNase I "footprint" that spans 18- of the 90-base-pair minimal oris sequence. The oris binding protein may, therefore, be analogous to other origin-specific binding proteins that are required for the initiation of viral and chromosomal DNA replication.