Open AccessIsolation of point mutations in bacteriophage Mu attachment regions cloned in a lambda::mini-Mu phage.
Author(s) -
Richard P. Burlingame,
Mark G. Obukowicz,
Debra L. Lynn,
Michael J. A. Howe
Publication year - 1986
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.83.16.6012
Subject(s) - lambda phage , point mutation , biology , mutant , base pair , bacteriophage , genetics , microbiology and biotechnology , dna , mutagenesis , lysogenic cycle , mutation , gene , escherichia coli
Twenty-one derivatives of a lambda::mini-Mu phage containing point mutations in the Mu attachment regions were isolated after mutD mutagenesis and selection for relief from Mu-specific replicative interference of lambda growth. DNA sequence analysis revealed that the single left-end mutant had suffered a T----C transition at position 1 of the Mu sequence, while the remaining 20 right-end mutants contained single base-pair insertions or deletions within the terminal 19 base pairs. A genetic assay showed that the right-end mutations revealed by sequencing were necessary for relief of the replicative inhibition of lambda growth. The properties of these mutants suggest that the terminal 2-base-pair and subterminal 8-base-pair inverted repeats are important for Mu-specific replicative transposition.