Open AccessDifferentiation-inducing factor purified from conditioned medium of mitogen-treated spleen cell cultures stimulates bone resorption.
Author(s) -
Etsuko Abe,
Hirofumi Tanaka,
Yoshiko Ishimi,
Chisato Miyaura,
Tomohiro Hayashi,
Hiroshi Nagasawa,
Mikio Tomida,
Yuri Yamaguchi,
Motoo Hozumi,
Toshio Suda
Publication year - 1986
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.83.16.5958
Subject(s) - spleen , concanavalin a , growth factor , resorption , macrophage colony stimulating factor , microbiology and biotechnology , tumor necrosis factor alpha , bone resorption , chemistry , biology , macrophage , endocrinology , immunology , biochemistry , in vitro , receptor
Spleen cells treated with mitogens produce a potent bone-resorbing factor called osteoclast-activating factor (OAF). To examine the relationship between the bone-resorbing factor and other protein factors produced by spleen cells, the colony-stimulating factor (CSF), the differentiation-inducing factor (DIF), the macrophage fusion factor (MFF), and the macrophage growth factor (MGF) were purified from 2.68 liters of conditioned medium of mouse spleen cell cultures treated with concanavalin A. Purification was performed successively by DEAE-cellulose, Blue Sepharose, and Sephadex G-150 column chromatography and high-pressure liquid chromatography (HPLC). The DIF was successfully separated from CSF and MGF on HPLC. CSF coincided with MGF on HPLC, but MFF disappeared before application to HPLC. Only the DIF exhibited bone-resorbing activity, whereas CSF and MGF did not. The DIFs purified from L929 cells and Ehrlich ascites tumors similarly exhibited bone-resorbing activity. The DIFs purified from spleen cells and Ehrlich ascites tumor cells exhibited neither interleukin 1 (IL-1) activity nor tumor necrosis factor (TNF) activity, though the unfractionated conditioned medium from spleen cells did exhibit them. In the light of recent reports that IL-1 beta and TNF also stimulate bone resorption, the term OAF should refer to a generic activity rather than a single factor.