Open AccessHuman chromosome 7 carries the beta 2 interferon gene.
Author(s) -
Pravinkumar B. Sehgal,
Asher Zilberstein,
R M Ruggieri,
L T May,
Anne C. FergusonSmith,
Doris L. Slate,
Michel Revel,
F.H. Ruddle
Publication year - 1986
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.83.14.5219
Subject(s) - microbiology and biotechnology , southern blot , biology , ecori , complementary dna , northern blot , gene , somatic cell , nucleic acid thermodynamics , interferon , chromosome , gene mapping , dna , gene expression , hybridization probe , genetics , restriction enzyme , rna
A cDNA clone (pAE20-4) corresponding to the 1.3-kilobase human beta 2 interferon mRNA was used as a probe in blot-hybridization experiments of DNA from a panel of human-rodent somatic cell hybrids containing overlapping subsets of human chromosomes. The DNA hybridization experiments showed that the human beta 2 interferon gene is located on human chromosome 7. This assignment is consistent with previous experimental data in which the expression of the translationally active 1.3-kilobase beta 2 interferon mRNA was assayed in various somatic cell hybrids. Blot-hybridization experiments using DNA from different human cell strains and cell lines reveal distinct EcoRI restriction fragment length polymorphisms of the human beta 2 interferon gene.
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