nu 1, a Mr 43,000 component of postsynaptic membranes, is a protein kinase. | Zendy

Adrienne S. Gordon | Zendy; Dale Milfay | Zendy

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nu 1, a Mr 43,000 component of postsynaptic membranes, is a protein kinase.

Author(s) -

Adrienne S. Gordon,

Dale Milfay

Publication year - 1986

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.83.12.4172

Subject(s) - torpedo , membrane , acetylcholine receptor , postsynaptic potential , chemistry , biochemistry , biophysics , monoclonal antibody , receptor , biology , antibody , immunology

Acetylcholine receptor-enriched membranes from the electric organ of Torpedo californica show a major band at Mr 43,000 on NaDodSO4/polyacrylamide gels. This band is composed of three polypeptides: nu 1, nu 2, and nu 3. Polypeptide nu 1 has been found to be localized exclusively at the innervated face of the electrocyte and at the neuromuscular junction in rat muscle. We show here that monoclonal antibody to nu 1 precipitates a radioactive Mr 43,000 polypeptide from detergent-solubilized extracts of Torpedo membranes covalently labeled with periodate-oxidized [alpha-32P]ATP. The monoclonal antibody also precipitates protein kinase activity from neutralized pH 11 extracts of the acetylcholine receptor-rich membranes. These data suggest that nu 1 is a postsynaptic membrane protein kinase.

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