Open AccessYeast RPO41 gene product is required for transcription and maintenance of the mitochondrial genome.
Author(s) -
Arno L. Greenleaf,
Jeffrey L. Kelly,
I. R. Lehman
Publication year - 1986
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.83.10.3391
Subject(s) - biology , gene , transcription (linguistics) , mitochondrial dna , microbiology and biotechnology , saccharomyces cerevisiae , genome , genetics , gene product , yeast , rna polymerase , rna , gene expression , philosophy , linguistics
A 4-kilobase DNA fragment carried by a recombinant lambda gt11 bacteriophage appears to contain most of the coding information for the 145-kDa subunit of the Saccharomyces cerevisiae mitochondrial RNA polymerase. The RPO41 gene is located on chromosome VI, as determined by hybridization to electrophoretically separated yeast chromosomes. Hybridization and gene disruption/replacement experiments show that the RPO41 gene exists in a single copy and that its product is required for transcription and maintenance of the mitochondrial genome.