Isolation of the gene and hypothalamic cDNA for the common precursor of gonadotropin-releasing hormone and prolactin release-inhibiting factor in human and rat. | Zendy

John P. Adelman | Zendy; Anthony J. Mason | Zendy; Joel S. Hayflick | Zendy; P. H. Seeburg | Zendy

Open Access

Isolation of the gene and hypothalamic cDNA for the common precursor of gonadotropin-releasing hormone and prolactin release-inhibiting factor in human and rat.

Author(s) -

John P. Adelman,

Anthony J. Mason,

Joel S. Hayflick,

P. H. Seeburg

Publication year - 1986

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.83.1.179

Subject(s) - complementary dna , biology , gonadotropin releasing hormone , prolactin , gene , peptide sequence , nucleic acid sequence , amino acid , homology (biology) , messenger rna , microbiology and biotechnology , exon , peptide , gonadotropin , hormone , biochemistry , luteinizing hormone

Cloned cDNAs encoding the precursor protein for gonadotropin-releasing hormone (Gn-RH) and prolactin release-inhibiting factor (PIF) were isolated from libraries derived from human and rat hypothalamic mRNA. Nucleotide sequence analyses predict precursor proteins of 92 amino acids for both species and show identity between the human placental and human hypothalamic precursor proteins. Whereas the Gn-RH peptide structure is completely conserved in human and rat, the PIF domain of the precursor displays 70% interspecies homology. Genomic analyses revealed the presence of a single Gn-RH-PIF gene in human and rat containing sequences corresponding to the cDNA distributed across four exons.

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