Open AccessDetection in extracts of bovine brain of lipophilic complexes of sulfate esters of cholesterol and beta-sitosterol.
Author(s) -
V. V. K. Prasad,
Ezzat El-Maraghy,
Laura Ponticorvo,
Seymour Lieberman
Publication year - 1985
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.82.9.2657
Subject(s) - sterol , chemistry , cholesterol , yield (engineering) , methanol , pyridine , chromatography , biochemistry , conjugate , sulfate , organic chemistry , mathematical analysis , materials science , mathematics , metallurgy
Evidence indicating that there exist in bovine brains hitherto-unrecognized lipophilic conjugates of sterol sulfates is presented. These conjugates are soluble in nonpolar solvents and, when heated in methanol containing pyridine, yield polar sterol conjugates. These polar substances have the chromatographic mobility of sterol sulfates and are cleaved to free sterols when subjected to a solvolytic process known to be specific for sulfate esters. The brain sterols that have been identified in this way are cholesterol and beta-sitosterol.