Open AccessMultiply drug-resistant human KB carcinoma cells have decreased amounts of a 75-kDa and a 72-kDa glycoprotein.
Author(s) -
Nancy Richert,
Shinichi Akiyama,
Donglai Shen,
Michael M. Gottesman,
Ira Pastan
Publication year - 1985
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.82.8.2330
Subject(s) - glycoprotein , wheat germ agglutinin , cell culture , microbiology and biotechnology , biology , vinblastine , p glycoprotein , methionine , biochemistry , molecular mass , drug resistance , multiple drug resistance , lectin , amino acid , genetics , chemotherapy , enzyme
Human KB carcinoma cells were selected in sequential steps for resistance to colchicine and found to be cross-resistant to multiple drugs, including vinblastine, adriamycin, and actinomycin D. Compared with the parental line, the multiply resistant cells have decreased amounts of two [35S]methionine-labeled proteins with apparent molecular masses of 75 and 72 kDa. These proteins reappear in a revertant, drug-sensitive cell line. Both proteins are labeled with [14C]glucosamine and are retained on a wheat germ agglutinin-agarose column, indicating that they are glycoproteins. These data suggest that in this human cell line, these two glycoproteins can serve as a marker of the multiple drug-resistance phenotype and may play a role in its etiology.
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