Open AccessPossible involvement of human D minigenes in the first complementarity-determining region of kappa light chains.
Author(s) -
Pojen P. Chen,
Elvin A. Kabat,
Tai Te Wu,
Sherman Fong,
Dennis A. Carson
Publication year - 1985
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.82.7.2125
Subject(s) - kappa , homology (biology) , genetics , complementarity (molecular biology) , complementarity determining region , biology , immunoglobulin light chain , recombination signal sequences , gene , nucleic acid sequence , microbiology and biotechnology , recombination , peptide sequence , recombination activating gene , mathematics , antibody , geometry
The nucleotide sequences of the complementary strands of two human diversity region (D) minigenes, D2 and D4, show stretches of homology with two human variable region kappa chain (V kappa) genes, NG9 and HK101, respectively, in the first complementarity-determining region. In one V kappa sequence, the homology includes the 5' flanking region of D minigenes, which may comprise a recombinase recognition signal. It is thus conceivable that gene conversions involving D minigenes may contribute to V kappa diversity.