Open AccessRibosomal RNA transcription: proteins and DNA sequences involved in preinitiation complex formation.
Author(s) -
Calvin T. Iida,
Preecha Kownin,
Marvin R. Paule
Publication year - 1985
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.82.6.1668
Subject(s) - transcription factor ii f , transcription preinitiation complex , transcription factor ii e , general transcription factor , transcription factor ii a , transcription factor ii b , transcription factor ii d , rna polymerase ii , eukaryotic transcription , biology , rna polymerase ii holoenzyme , microbiology and biotechnology , transcription (linguistics) , transcription bubble , promoter , genetics , rna polymerase , rna , gene expression , gene , linguistics , philosophy
An in vitro transcription system consisting of partially purified transcription initiation factor(s) and purified RNA polymerase I from Acanthamoeba castellanii was used to study the mechanism of faithful initiation of ribosomal RNA transcription. Formation of a preinitiation complex between one or several auxiliary transcription proteins and the DNA template in the absence of RNA polymerase I was demonstrated. A series of 3'- and 5'-deletion mutants of the template was used in prebinding competition experiments and provided evidence for three distinct functional regions of the promoter: core motif A interacts with the transcription initiation factor(s) and is required for faithful transcription; the start motif is required for transcription, but it can be deleted without affecting the binding of transcription initiation factor(s); and motif B stabilizes preinitiation complex formation (in addition to core motif A), but it is dispensable for faithful initiation of transcription.