Open AccessCloning and nucleotide sequence determination of the Clostridium pasteurianum ferredoxin gene.
Author(s) -
Mary C. Graves,
Guy T. Mullenbach,
Jesse C. Rabinowitz
Publication year - 1985
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.82.6.1653
Subject(s) - nucleic acid sequence , biology , pbr322 , ferredoxin , plasmid , microbiology and biotechnology , clostridium , dna , gene , genetics , genomic dna , sequence analysis , peptide sequence , biochemistry , enzyme , bacteria
We have constructed a library of Clostridium pasteurianum DNA cloned in the plasmid pBR322. Based on the known amino acid sequence for C. pasteurianum ferredoxin, a 64-fold degenerate heptadecanucleotide pool was synthesized. This mixed probe hybridized to two clones which were shown to contain greater than 6 kilobase pairs of the same genomic DNA. Sequence analysis of a common Sau3A1 0.6-kilobase-pair fragment revealed that it contains the information for the apoferredoxin structural gene. According to the DNA sequence, the only post-translational processing of this small apoprotein is the hydrolysis of the initiator methionine. Putative transcription and translation start and stop signals are present within the sequence.