Open AccessCharacterization of a Q subregion gene in the murine major histocompatibility complex.
Author(s) -
Lorraine Flaherty,
Kathleen Dibiase,
Michael A. Lynes,
J. G. Seidman,
Ofra Weinberger,
Eugene M. Rinchik
Publication year - 1985
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.82.5.1503
Subject(s) - biology , gene , southern blot , microbiology and biotechnology , mutant , genetics , restriction enzyme , major histocompatibility complex , restriction fragment length polymorphism , dna , restriction fragment , structural gene , polymerase chain reaction
We have used restriction enzyme digests, Southern blot analysis, and gene transfer experiments to identify a class I gene in the Q subregion of the murine major histocompatibility complex. By comparisons of class I genes from Q congeneic strains, five restriction fragment length polymorphisms were identified. Further studies of mutant (Qa-2-) and wild-type (Qa-2+) BALB/c sublines indicated that at least part of the structural or regulatory gene controlling a Q subregion antigen resides on a 3.7-kilobase Xba I DNA fragment and is absent in all tested Qa-2- strains. The spontaneously occurring Qa-2- BALB/cBy mutant appears to have an extensive deletion in this region. The identity of this gene was confirmed by gene transfer experiments as well as by the use of a single-copy probe.