Characterization of a Q subregion gene in the murine major histocompatibility complex. | Zendy

L Flaherty | Zendy; Kathleen DiBiase | Zendy; Michael A. Lynes | Zendy; J G Seidman | Zendy; O Weinberger | Zendy; Eugene M. Rinchik | Zendy

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Characterization of a Q subregion gene in the murine major histocompatibility complex.

Author(s) -

L Flaherty,

Kathleen DiBiase,

Michael A. Lynes,

J G Seidman,

O Weinberger,

Eugene M. Rinchik

Publication year - 1985

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.82.5.1503

Subject(s) - biology , gene , southern blot , microbiology and biotechnology , mutant , genetics , restriction enzyme , major histocompatibility complex , restriction fragment length polymorphism , dna , restriction fragment , structural gene , polymerase chain reaction

We have used restriction enzyme digests, Southern blot analysis, and gene transfer experiments to identify a class I gene in the Q subregion of the murine major histocompatibility complex. By comparisons of class I genes from Q congeneic strains, five restriction fragment length polymorphisms were identified. Further studies of mutant (Qa-2-) and wild-type (Qa-2+) BALB/c sublines indicated that at least part of the structural or regulatory gene controlling a Q subregion antigen resides on a 3.7-kilobase Xba I DNA fragment and is absent in all tested Qa-2- strains. The spontaneously occurring Qa-2- BALB/cBy mutant appears to have an extensive deletion in this region. The identity of this gene was confirmed by gene transfer experiments as well as by the use of a single-copy probe.

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