Open Access"Retroposon" insertion into the cellular oncogene c-myc in canine transmissible venereal tumor.
Author(s) -
Nurit Katzir,
Gideon Rechavi,
Justus B. Cohen,
Tamar Unger,
Frida Brok Simoni,
Shraga Segal,
Dan Cohen,
David Givol
Publication year - 1985
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.82.4.1054
Subject(s) - biology , microbiology and biotechnology , transposable element , dna , retroposon , genetics , exon , direct repeat , repeated sequence , inverted repeat , insertion sequence , homology (biology) , base pair , southern blot , gene , genome , base sequence
We examined by Southern blotting the state of the cellular oncogene c-myc in the dog transmissible venereal tumor. The tumor DNA contains a 16.8-kilobase pair (kbp) rearranged c-myc fragment in addition to the normal 15-kbp and 7.5-kbp fragments. We compared the structure of the cloned rearranged c-myc (re-myc) with that of a cloned normal c-myc and found that the rearrangement was due to the insertion of a 1.8-kbp DNA upstream to the first exon of c-myc. The inserted DNA is flanked by 10-base-pair direct repeats and contains a dA-rich tail, suggesting its origin from mRNA. Partial sequence of the inserted element showed 62% homology with the primate interdispersed Kpn I repetitive element. These results provide an example for the behavior of repetitive DNA sequences like the Kpn I family, as movable elements that can transpose nearby to oncogenes or other structural genes and perhaps affect their activity.