Involvement of ribosomal protein L7/L12 in control of translational accuracy. | Zendy

Leif A. Kirsebom | Zendy; Leif A. Isaksson | Zendy

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Involvement of ribosomal protein L7/L12 in control of translational accuracy.

Author(s) -

Leif A. Kirsebom,

Leif A. Isaksson

Publication year - 1985

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.82.3.717

Subject(s) - ribosome , proofreading , ribosomal protein , transfer rna , biology , genetics , ribosomal rna , protein biosynthesis , mutant , translation (biology) , locus (genetics) , messenger rna , gene , rna , polymerase

The effects of two mutations, which map at the rplL locus and both give a changed 50S ribosomal protein L7/L12, were studied. Both mutations are associated with an increased misreading of all three nonsense codons in vivo and ribosomes from the mutants give an increased misreading of the phenylalanine codon UUU by tRNALeu in vitro. The rplL-associated misreading in vitro is not limited to a particular type of mRNA or tRNA. Results from a translational proofreading assay, using mutant ribosomes, suggest that protein L7/L12 is involved in the control of translational accuracy by contributing to the efficiency of a translational proofreading step(s).

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