The herpes simplex virus amplicon: analyses of cis-acting replication functions. | Zendy

Richard R. Spaete | Zendy; N Frenkeĺ | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

The herpes simplex virus amplicon: analyses of cis-acting replication functions.

Author(s) -

Richard R. Spaete,

N Frenkeĺ

Publication year - 1985

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.82.3.694

Subject(s) - amplicon , herpes simplex virus , biology , virology , genome , virus , viral replication , helper virus , origin of replication , rolling circle replication , dna , dna replication , genetics , gene , polymerase chain reaction

Previous studies have shown that defective virus vectors (amplicons) derived from herpes simplex viruses could be efficiently propagated in virus stocks in the presence of trans-acting helper virus functions. The present study established that two separate cis-acting functions--a DNA replication origin and a cleavage/packaging signal--are required for amplicon propagation. Using deleted derivatives of cloned amplicons, we mapped one of the viral DNA replication origins (ori-2 or oriL) at coordinate 0.422 of the standard HSV-1 genome and at an equivalent position within the HSV-2 genome.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research