Open AccessExpression of the cytokeratin endo A gene during early mouse embryogenesis.
Author(s) -
Philippe Duprey,
Dominique Morello,
Marc Vasseur,
Charles Babinet,
Hubert Condamine,
Philippe Brûlet,
François Jacob
Publication year - 1985
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.82.24.8535
Subject(s) - blastocyst , biology , endoderm , inner cell mass , embryo , messenger rna , in situ hybridization , microbiology and biotechnology , embryogenesis , gene expression , cytokeratin , cellular differentiation , andrology , gene , genetics , immunology , immunohistochemistry , medicine
Expression of cytokeratin endo A has been analyzed during mouse blastocyst formation and embryonal carcinoma cell differentiation. To study the regulation of endo A expression, nuclease S1 mapping experiments have been performed on RNA extracted from two-cell to 7.5-day embryos. Low levels of endo A mRNA begin to be detectable in eight-cell embryos. The amount of this mRNA increases at the blastocyst stage, suggesting that endo A expression is regulated at the mRNA level during blastocyst formation. At this stage, in situ hybridization studies show that endo A mRNA is present in the trophectoderm but not in the inner cell mass. In 7.5-day embryos, endo A mRNAs are also detectable in the endoderm layer and in the amnion.