Open AccessMultistep transformation by defined fragments of herpes simplex virus type 2 DNA: oncogenic region and its gene product.
Author(s) -
Yoshinobu Hayashi,
Tsuyoshi Iwasaka,
Cynthia C. Smith,
Laure Aurelian,
George K. Lewis,
Paul O. P. Ts’o
Publication year - 1985
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.82.24.8493
Subject(s) - microbiology and biotechnology , bamhi , ecori , biology , hindiii , transfection , monoclonal antibody , herpes simplex virus , dna , immunofluorescence , antibody , cell culture , virology , virus , restriction enzyme , biochemistry , genetics
Diploid Syrian hamster embryo cells transfected with Bgl II C fragment of herpes simplex virus type 2 DNA acquired a neoplastic phenotype. Cultures transfected with its left-hand 64% subclone EcoRI/HindIII fragment AE (0.419-0.525 map unit) grew into established but nontumorigenic lines. Transfection of EcoRI/HindIII AE-immortalized cells with a 4.4-kilobase Sac I/BamHI subfragment within BamHI E (0.554-0.584 map unit; overlaps the right-hand 16% of Bgl II C) converted them to tumorigenicity. The 4.4-kilobase subfragment encodes a 144-kDa protein immunologically and structurally similar to an infected cell protein designated ICP 10. DNA extracted from cells transformed with the 4.4-kilobase subfragment exhibited discrete hybridizing bands homologous to BamHI E fragment. Monoclonal antibody to ICP 10 precipitated a 144-kDa protein from the transformed cells and stained them in immunofluorescence. A tumor derivative established with the transformed cells did not stain with this antibody, but approximately equal to 25% of the cells stained with a monoclonal antibody to c-myc protooncogene products.