Open AccessMonoclonal antibodies to human vitamin D-binding protein.
Author(s) -
Eric A. Pierce,
Margaret C. Dame,
Roger Bouillon,
Hugo Van Baelen,
Hector F. DeLuca
Publication year - 1985
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.82.24.8429
Subject(s) - immunoprecipitation , monoclonal antibody , vitamin d binding protein , antibody , binding protein , protein g , vitamin , microbiology and biotechnology , biochemistry , protein a/g , retinol binding protein , biology , plasma protein binding , protein a , chemistry , recombinant dna , retinol , immunology , gene , fusion protein
Monoclonal antibodies to vitamin D-binding protein isolated from human serum have been produced. The antibodies obtained have been shown to be specific for human vitamin D-binding protein by three independent assays. The antibodies recognize human vitamin D-binding protein specifically in an enzyme-linked immunosorbent assay. Human vitamin D-binding protein is detected specifically in both pure and crude samples by a radiometric immunosorbent assay (RISA) and by an immunoprecipitation assay. The anti-human vitamin D-binding protein antibodies cross-react with monkey and pig vitamin D-binding protein, but not with vitamin D-binding protein from rat, mouse, or chicken, as determined by the RISA and immunoprecipitation assays.