Open AccessNonconsensus branch-site sequences in the in vitro splicing of transcripts of mutant rabbit beta-globin genes.
Author(s) -
Richard A. Padgett,
Maria M. Konarska,
Markus Aebi,
Horst Hornig,
Charles Weissmann,
Phillip A. Sharp
Publication year - 1985
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.82.24.8349
Subject(s) - primer extension , rna splicing , biology , gene , mutant , microbiology and biotechnology , genetics , rna , globin , consensus sequence , intron , peptide sequence
Mutants of the rabbit beta-globin gene lacking the natural site of branch formation in the second intervening sequence have been analyzed for in vitro splicing activity. RNAs transcribed from these mutants were spliced, via lariat formation, at a reduced rate compared to wild-type RNA. The sites of branch formation were mapped by direct RNA analysis and primer-extension analysis. The sequences at the branch sites in the three mutants examined did not conform to the previously determined consensus sequence, nor were the 5' splice sites and branch sites complementary.