Identification of a high molecular weight polypeptide that may be part of the circadian clockwork in Acetabularia | Zendy

Romana Hartwig | Zendy; Michal R. Schweiger | Zendy; Regina Schweiger | Zendy; HansGeorg Schweiger | Zendy

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Identification of a high molecular weight polypeptide that may be part of the circadian clockwork in Acetabularia

Author(s) -

Romana Hartwig,

Michal R. Schweiger,

Regina Schweiger,

HansGeorg Schweiger

Publication year - 1985

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.82.20.6899

Subject(s) - acetabularia , cycloheximide , circadian rhythm , biology , protein biosynthesis , chloramphenicol , ribosome , euglena gracilis , darkness , endogeny , chloroplast , biochemistry , microbiology and biotechnology , rna , botany , endocrinology , gene , antibiotics

In the chloroplast fraction of the unicellular and uninucleate green algaAcetabularia , we have detected aMr ≈230,000 protein (p230) whose synthesis exhibits a pronounced endogenous diurnal rhythm. As judged by scanning densitometry of fluorographs of NaDodSO4 /polyacrylamide gels, the synthesis of other proteins in the same fraction was independent of the time in the cycle. The incorporation of [35 S]methionine into p230 was completely inhibited by cycloheximide, whereas chloramphenicol had no effect. This strongly suggests that p230 is translated on 80S ribosomes. Eighthour periods of exposure to cycloheximide produced a shift in the phase of the oscillation of p230 synthesis. The results are consistent with the hypothesis that p230 is essential for expression of circadian rhythms inAcetabularia .

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