Repair of defined single base-pair mismatches in Escherichia coli | Zendy

Christiane Dohet | Zendy; Robert E. Wagner | Zendy; Miroslav Radman | Zendy

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Repair of defined single base-pair mismatches in Escherichia coli

Author(s) -

Christiane Dohet,

Robert E. Wagner,

Miroslav Radman

Publication year - 1985

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.82.2.503

Subject(s) - escherichia coli , bacteriophage , base pair , biology , dna , genetics , dna repair , transfection , enterobacteriaceae , microbiology and biotechnology , mutant , gene

Heteroduplexes with single base-pair mismatches of known sequence were prepared by annealing separated strands of bacteriophage lambda DNA and used to transfect Escherichia coli. Each of the eight possible single base-pair mismatches was constructed. Genetic analysis of the progeny phages obtained from transfected bacteria indicates that the E. coli mismatch repair system does not recognize (or does not repair) all single base-pair mismatches with equal efficiency. In particular, the A.G, C.T, and C.C mismatches appear to be less repaired than any others. The mutator character of mismatch repair-deficient mutants suggests that such unrepaired mismatches should occur infrequently during E. coli DNA replication.

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