Cloning, characterization, and sequence of the yeast DNA topoisomerase I gene. | Zendy

C Thrash | Zendy; Alan T. Bankier | Zendy; B. G. Barrell | Zendy; Rolf Sternglanz | Zendy

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Cloning, characterization, and sequence of the yeast DNA topoisomerase I gene.

Author(s) -

C Thrash,

Alan T. Bankier,

B. G. Barrell,

Rolf Sternglanz

Publication year - 1985

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.82.13.4374

Subject(s) - gene , biology , genetics , plasmid , in vitro recombination , dna , molecular cloning , saccharomyces cerevisiae , genomic dna , yeast , cloning (programming) , dna sequencing , microbiology and biotechnology , peptide sequence , computer science , programming language

The structural gene for yeast DNA topoisomerase I (TOP1) has been cloned from two yeast genomic plasmid banks. Integration of a plasmid carrying the gene into the chromosome and subsequent genetic mapping shows that TOP1 is identical to the gene previously called MAK1. Seven top1 (mak1) mutants including gene disruptions are viable, demonstrating that DNA topoisomerase I is not essential for viability in yeast. A 3787-base-pair DNA fragment including the gene has been sequenced. The protein predicted from the DNA sequence has 769 amino acids and a molecular weight of 90,020.

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